Peony (Paeonia lactiflora Pall., Paeoniaceae) has been in cultivation in China for thousands of years, and have been grown as garden and outdoor cut flower plants in Europe and the US for many years. Cut flowers were, however, available only for a few weeks a year during the natural flowering season in late spring. Until recently, however, very little was known on the flowering physiology of the plant. We have found that flower bud initiation starts after the old leaves senescence in the summer and continues until late autumn when they become dormant. Release from dormancy requires a period of low temperatures, and can be accelerated by GA treatment. After the release from dormancy the plants may start growing and blooming under mild-warm temperatures (Wilkins and Halevy 1985; Byrne and Halevy 1986). This basic information enabled the development of a practical method for extending the flowering season and obtaining cut flower production in the winter, 2–3 months before the natural flowering season (Halevy et al. 1995). Plants are grown under ambient natural cold temperatures of the early winter. After sufficient cold units are accumulated, the structures are covered with polyethylene at mid-winter and the plants are drenched with GA solution. Sprouting and flowering soon follow.
The introduction and improvement of
this "new crop" is actually
developing new horticultural techniques for flowering control of a very
old ornamental plant. One of the obstacles of rapid development of
peony as a commercial crop is the slow rate of natural propagation by
division of crowns. We are now developing a tissue culture propagation
method that should solve this problem.
Dear colleagues,
After various years of hard job finally I have success to obtain Tree
Peony in vitro. Plants obtained are good, robust and with a radical
apparatus constituted from 4/7 short and large roots/plant. The
acclimatization has given to good result with a low percentage of dead plants.
But the rooting plants obtained in vitro is alone 80%, therefore it
would appeal to you if someone has worked on this plant and has obtained
of the turning out bonds. Regards.
Nives Gimelli
Vivai Battistini
Laboratorio micropropagazione
via Emilia, 3551
47020-Diegaro di Cesena (FC)
Italy
www.vivaibattistini.com
microvitro@libero.it
Eleonora Gabryszewska. Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100 Skierniewice, Poland
The
influence of ABA, fluridone, carbohydrates (sucrose, glucose,
fructose), light quality (white, blue, red) and temperature (4 C,
21 C)
on the growth and dormancy of peony cv. Jadwiga shoots were
investigated. Dormant and non-dormant shoots were cultured on the
Murashige and Skoog (1962) medium with or without cytokinins
(BAP+2iP+kinetin+TDZ).
The application of ABA with cytokinins inhibited shoot and leaf
formation both in non-dormant and in dormant shoots. However,
fluridone, an inhibitor of ABA-synthesis, promoted leaf and shoot
production. The activity of fluridone was modified by light quality;
the highest number of leaves and shoots were found on the explants
grown under the red light. The influence of carbohydrates on the shoot
formation and growth was modified by the light quality also. The blue
light increased the number of shoots and leaves produced by non-dormant
and dormant shoots on the medium with sucrose or fructose. However, the
white and red lights stimulated the multiplication rate and leaf
formation in the presence of glucose. The shoot culture stored for
12-16 weeks at the temperature of +4 C tended to have a higher
multiplication rate than the culture
maintained at the continuous
temperature of 21 C.
ISHS Acta Horticulturae 560: IV International
Symposium on In Vitro Culture and Horticultural Breeding
| Author: | E. Gabryszewska |
| Keywords: | peony, ABA, fluridone, light quality, in vitro, dormancy |
| Abstract:
The influence of ABA 1 mg l-1, fluridone 1 mg l-1 and light quality (white, red, blue) on the growth and dormancy of non-dormant and dormant peony cv. Jadwiga shoots grown on the media without or with cytokinins (BAP+2iP+kinetin+thidiazuron) were investigated. In the shoots that became dormant, the rate of growth and the rate of multiplication were reduced. Also, senescence and death of the leaves took place. The application of ABA or fluridone to the cytokinin-free medium had no effect on the number of axillary shoots but influenced the leaf formation on non-dormant and dormant shoots grown under all light qualities. The addition of ABA with cytokinins inhibited the leaf production in non-dormant as well as dormant shoots. Fluridone, an inhibitor of ABA-synthesis, applied during culture, promoted leaf formation and axillary shoot production. The activity of fluridone in leaf and shoot formation was modified by light quality. The highest number of leaves and shoots were found on the explants grown under red light both on non-dormant and dormant shoots. The inhibition of leaf formation by ABA and the opposite effect of fluridone in this processes suggest that development of dormancy in tissue-cultured peony shoots can be caused by ABA. |
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