pH 5.8
+ MS + KH2PO4 + CH 
AX clusters initiation ADV embryogenesis induction
0.1 NAA + 0.2
TDZ
40 mg/L
2,4-D
AX clusters
production
ADV embryogenesis production
2 BAP + 0.2 TDZ + 0.03 NAA 20 mg/L 2,4-D + 0 or 0.5 BAP mg/L
2
BAP + 0.4 TDZ + 0.05
NAA
...........................................................
VB® or GX® +
Gelrite 1.7 g/L + 15 g/L
Glucose
AX clusters production ADV embryogenesis production
1 BAP + 0.1 TDZ + 0.03 NAA 5 mg/L 2,4-D + 0.2 BAP or 0.5 2iP
http://www.cropsoil.uga.edu/homesoybean/somprot.htm
http://www.cropsoil.uga.edu/homesoybean/embryo.htm
http://www.cropsoil.uga.edu/~parrottlab/Tour/building.html
- Subject: Re: meristematic vs embryogenic callus
- From: Wayne Parrott <wparrott@ARCHES.UGA.EDU>
- Date: Fri, 15 Dec 2000 08:49:41 -0500
- Reply-To: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>
- Sender: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>
At 10:07 AM 12/15/00 +1000, you wrote:
>I think term "embryogenic callus" is not appropriate. What we call as
>"embryogenic callus" is not a callus but a cluster of proembryos. While
>callus is a result of plant cells multiplying in a disorganized way.
>Should we look for some appropriate terminology?
>Poonam
Embryogenic callus *is* an appropriate term when it truly consists of
callus that gives rise to embryos. A cluster of proembryos or
globular-stage embryos giving rise to additional embryos has been termed
proliferative embryos or repetitive embryos, among other things. I still
prefer 'embryogenic tissue'
Wayne Parrott
Dept. Crop & Soil Sciences
University of Georgia
Athens, GA 30602-7272
http://www.cropsoil.uga.edu/~parrottlab
MICROPROPAGATION OF FERNS: geocities.com/sagey_ph/fernculture.html