New
low
salt medium Joan
EC 1413 µS/cm
Moss Koz Daria Utah
Joan or S4
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NH4NO3 0 0 0 100 300
KNO3 300 200 300 200 500
Ca(NO3)2.4H2O 700 500 500 200 200
MgSO4.7H2O 300 200 250 100 100
KH2PO4 200 200 170 100 100
K2SiO3 30 0 30 20 20
FeEDDHA 20 20 22 12 20
Micro Daria 2 ml Utah 2 ml Utah 2 ml Dar 2 ml
Inositol 50 0 0 0
vit-Rosa 1 ml 0.2 B1 0.2 B1 0.2 B1
CH-Tryptone 200 0 0 0
Glucose 5000 0 0 0
pH 5.2 5.7 5.7 5.7
Micro Daria Daria Phal(3.12.07)
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H3BO3 200 2 2
MnSO4.1H2O 400 4 3
ZnNa2EDTA 200 2 2
Na2MoO4.2H2O 10 0.10 0.05
CuSO4.5H2O 5 0.05 0.05
CoCl2.6H2O 2 0.02 0.02
NiCl2.6H2O 1 0.01 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
Vit Rosa Vit Orch*
------------------------------------------------------------
Thiamine B1 100 1.0 0.5
Nicotinic acid NA 50 0.5 5.0
Pyridoxine B6 50 0.5 0.5
Glycine 200 2 2
Ca Pantothenate 200 2 2
Biotin, D(+) 2 0.02 0.05
Folic acid 50 0.5 0.5
H2O ad 100 *1 ml
CACTI RAPID MULTIPLICATION
Glucose 0 + 7000 PEG + 0.5 BAP + 0.03 NAA
EXPRESSIVE AUREOLE SPRAY
Glucose 0 + 3000 PEG + 10 GA3
Micro Rosa mg mg/l
======================================================
H3BO3 800 8
MnSO4.1H2O 1500 15
ZnNa2EDTA 1000 10
Na2MoO4.2H2O 25 0.25
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
Micro Holland (–Mn) Dicotyledone
======================================================
H3BO3 800 8
MnSO4.1H2O 800 8
ZnNa2EDTA 800 8
Na2MoO4.2H2O 20 0.20
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
Micro Holland (–Mn –B) Monootyledone
======================================================
H3BO3 300 3
MnSO4.1H2O 800 8
ZnNa2EDTA 800 8
Na2MoO4.2H2O 20 0.20
CuSO4.5H2O 10 0.10
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
KJ mg mg/l
======================================================
KJ 50 0.5
H2O ad 50 ml *0.5-0.3 ml
Composition of the Universal medium mg/l
Macronutrients
KNO3 849.2
KH2PO4 158.8
NaNo3 66.4
NH4H2PO4 17.5
NH4NO3 369.5
NaH2PO4 x H2O 73.4
Ca(NO3)2 x 4H2O 277.7
Na2HPO4 x 12 H2O 2.6
CaCl2 116.7
Na2SO4 x 10 H2O 112.7
MgSO4 x 7 H2O 342.3
(NH4)2SO4 88.8
KCl 149.4
NH4Cl 12.3
Micronutrients
MnSO4 x H2O 10.377
CoCl2 x 6 H2O 0.039
ZnSO4 x 7 H2O 5.523
FeEDTA 28.429
H3BO3 4.508
Na2EDTA x 2 H2O 30.667
KJ 0.603
AlCl3 x 6 H2O 0.004
CuSO4 x 5 H2O 0.066
NiCl2 x 6 H2O 0.003
Na2MoO4 x 2 H2O 0.159
Vitamins
Myo-inositol 132.023
Folic acid 0.175
Thiamine-HCl 1.575
Choline chloride 0.227
Nicotinamide 1.292
p-Amino benzoic acid 0.038
Pyridoxine-HCl 0.521
Riboflavin 0.032
Ca-D-pantothenate 0.616
Ascorbic acid 0.503
Biotin 0.053
Amino acids and other organic supplements
Glycin 3.073
L-Asparagine 10.400
L-Arginine 9.024
L-Methionine 0.955
L-Aspartic acid 5.727
L-Tyrosin 1.928
L-Cysteine 2.456
Casein hydrolysate 217.982
L-Glutamic acid 6.394
Urea 7.273
L-Glutamin 84.400
pH 5.6