pH 5.8            

DOROTA + 1% sucrose + 0.5 BAP + 0.03 TDZ + 0.03 NAA + GELRITE

½MS + KH2PO4 + 2% sucrose + 0.5 BAP + 0.03 TDZ + 0.03 NAA 

DT + 2% sucrose + 0.5 BAP + 0.03 TDZ + 0.03 NAA

IN VITRO PROPAGATION OF CAMPANULA ISOPHYLLA
Rael VARDJA, Tõnis VARDJA, Ao PAE  Campanula isophylla rūšys paprastai dauginamos auginiais, tačiau šiltnamiuose veislinius augalus stipriai pažeidžia fuzariozė. Parengta Campanula isophylla dauginim  in vitro sistema sveikai veislinei medžiagai gauti. Geriausi dauginimo rezultatai (proliferacijos koficientas / eksplanto kokybė) gauti pirmuose dviejuose variantuose Murasiges - Skugo terpeje, turinčioje 2% sacharozės, 2 mg/l benziladenino (BA) ir 0,1 mg/l indolilsviesto rugšties (ISR). BA pastoviai mažinta iki 0,5 mg/l. Visi eksplantai išleido šaknis MS terpėje su 2% sacharoze, be augimo reguliatorių. Geriausios šaknys susidarė modifikuotoje MS terpėje (1/2 NH4NO3), turinčioje 2% sacharozės, 0,1mg/l BA ir 0,5 mg/l ISR. Aklimatizavosi 85%.

Induction of adventitious shoots in vitro in Campanula carpatica 2004
Sriskandarajah Sridevy  Frello Stefan and  Serek Margrethe
Efficient protocols have been developed to induce adventitious shoots in different types of explants of Campanula carpatica Jacq. More than five shoots per explant developed on hypocotyls of 5-week-old seedlings after 2 weeks of culture. Hypocotyls produced twice as many shoots as the cotyledons. TDZ proved to be about 6 times more efficient than BA. NAA had to be added to the regeneration medium to obtain the optimal balance of auxin and cytokinin to induce shoot regeneration. Significant differences were noted between different growth regulator concentrations in their effects on shoot organogenesis. BA induced double the number of callus clumps as TDZ. Incubation of explants in the dark produced about 6 shoots per explant while those in the light produced about 2 shoots per explant. Explants derived from 5-week-old seedlings were five times more regenerative compared to those derived from 15-week-old seedlings. Explants from cv. White Uniform were more organogenic than those from cv. Blue Clip. Root segments were also found to form shoots when treated with CPPU.

Induction of adventitious shoots in vitro in Campanula carpatica 2001
Sriskandarajah S.; Frello S.; Serek M.  Incubation of explants in the dark produced about 6 shoots per explant while those in the light produced about 2 shoots per explant.

In vitro propagation of Campanula glomerata, ‘Acaulis’ from leaf blade explants 2002 
1 BAP + 0.01 NAA + light irradiance