LIQUID MEDIA & GELRITE
1600 - Last
modified May
12, 2005
DARIA
- MOSS
2-3 times faster growth rate
.
S4® mg/l mg/4l mg/8l
--------------------------------------------------------------
Citric Ac 20 80 160
NH4NO3 300 1200 2400
KNO3 500 2000 4000
Ca(NO3)2.4H2O 200 800 1600
MgSO4.7H2O 100 400 800
KH2PO4 100 400 800
FeEDDHA 20 80 160
KJ 0.1 0.4 ml 0.8 ml
Micro Pinus Liquid 2 ml 8 ml 16 ml
Vit Orchids 1 ml 4 ml 8 ml
PEG 6000 200 800 1600
CH,Tryptone 200 800 1600
L-Asparagine 200 800 1600
Inosit 25 100 200
Ade 10 40 80
Vit C 50
STS 2
Glucose 6-8 g
pH 5.5
H2O ad 1000 ml 400 ml 800 ml
S4© mg/l mg/4l mg/8l
--------------------------------------------------------------
Citric Ac 20 80 160
NH4NO3 300 1200 2400
KNO3 400 1600 3200
Ca(NO3)2.4H2O 200 800 1600
MgSO4.7H2O 100 400 800
KH2PO4 75 300 600
FeEDDHA 20 80 160
KJ 0.1 0.4 ml 0.8 ml
Micro Pinus Liquid 2 ml 8 ml 16 ml
Vit Orchids 1 ml 4 ml 8 ml
CH,Tryptone 200 800 1600
Inosit 25 100 200
Ade 10 40 80
Vit C 50
STS 2
Glucose 6 g
pH 5.5
H2O ad 1000 ml 400 ml 800 ml
ACER 2003 ½Knopp 1862 mg/L
--------------------------------
Citric acid 20
NH4NO3 100
KNO3 200
Ca(NO3)2.4H2O 500
MgSO4.7H2O 200
KH2PO4 200
FeEDDHA 20
KJ 0.1
Micro Pinus liquid 2 ml
Vit Rosa 1 ml
Inosit 25
Ade 10
CH-Tryptone 200
Vit C 50
STS 2
CM Cellulose ROTOR 4 g
Glucose 4-8 g
pH 5.8
H2O ad 1000 ml
.
VB® mg/l mg/4l mg/8l
-----------------------------------------------------------------
Citric Ac 100 300 600
NH4NO3 500 2000 4000
KNO3 1500 6000 12000
Ca(NO3)2.4H2O 500 2000 4000
MgSO4.7H2O 200 +100 600 1200
KH2PO4 200 800 1600
FeEDDHA 50 200 400
KJ 0.25 1 ml 2 ml
Micro Orchids Liquid 2 ml 8 ml 16 ml
Vit Orchids/Rosa/PLB 1 ml 4 ml 8 ml
[PEG 6000 200 800 1600]
CH,Tryptone 500 2000 4000
L-Asparagine 500 2000 4000
Inosit 100 400 800
Ade 25 100 200
Vit C 50
STS 2
CM Cellulose ROTOR 4 g
Sucrose 20 g or Glucose 15 g
pH 5.5
H2O ad 1,000 ml 800 ml 1600 ml
-----------------------------------------------------------------
* GOHA = 2 Kin + 1 IAA + 2 g Gerlite
.
VB© mg/l mg/4l mg/8l
--------------------------------------------------------------
Citric Ac 50 150 300
NH4NO3 400 1600 3200
KNO3 1500 6000 12000
Ca(NO3)2.4H2O 500 2000 4000
MgSO4.7H2O 200 800 1600
KH2PO4 150 600 1200
FeEDDHA 40 160 160
KJ 0.25 1 ml 2 ml
Micro Orchids Liquid 2 ml 8 ml 16 ml
Vit Orchids/Rosa/PLB 1 ml 4 ml 8 ml
CH,Tryptone 400 1600 3200
Inosit 100 400 800
Ade 20 80 160
Vit C 50
STS 2
CM Cellulose ROTOR 4 g
Sucrose 15-20 g or Glucose 12-15 g
pH 5.5
H2O ad 1,000 ml 800 ml 1600 ml
.
GX® mg/l mg/4l mg/8l
--------------------------------------------------------------
Citric Ac 100 400 800
NH4NO3 500 2000 4000
KNO3 1000 4000 8000
Ca(NO3)2.4H2O 1500 6000 12000
MgSO4.7H2O 500 2000 4000
KH2PO4 200 800 1600
FeEDDHA 50 200 400
KJ 0.5 2 ml 4 ml
Micro Holland 2 ml 8 ml 16 ml
Vit Orchids/Rosa/PLB 1 ml 4 ml 8 ml
CH,Tryptone 500 2000 4000
L-Asparagine 500 2000 4000
Inosit 100 400 800
Ade 25 100 200
Vit C 50
STS 2
Sucrose 20 g
pH 5.5
H2O ad 1,000 ml 800 ml 1600 ml
.
GX© mg/l mg/4l mg/8l
--------------------------------------------------------------
Citric Ac 100 400 800
NH4NO3 400 1600 3200
KNO3 1000 4000 8000
Ca(NO3)2.4H2O 1500 6000 12000
MgSO4.7H2O 400 1600 3200
KH2PO4 200 800 1600
FeEDDHA 50 200 400
KJ 0.5 2 ml 4 ml
Micro Holland 2 ml 8 ml 16 ml
Vit Orchids/Rosa/PLB 1 ml 4 ml 8 ml
CH,Tryptone 500 2000 4000
Inosit 100 400 800
Ade 25 100 200
Vit C 50
STS 2
Sucrose 20 g
pH 5.5
H2O ad 1,000 ml 800 ml 1600 ml
.
½LM mg/l mg/4l mg/8l
--------------------------------------------------------------
Citric Ac 100 400 800
NH4NO3 800 3200 6400
KNO3 1000 4000 8000
CaCl2.2H2O 10 40 800
MgSO4.7H2O 800 3200 6400
KH2PO4 200 800 1600
FeEDDHA 30 120 240
KJ 0.25 1 ml 2 ml
Micro Pinus Micro HOLLAND 2 ml 8 ml 16 ml
Vit Gamborg 1 ml 4 ml 8 ml
PEG 6000 250 1000 2000
CH,Tryptone or asparagine 400 1600 3200
Ala-GLN 50 200 400
Inosit 50 200 400
Ade 25 100 200
Sucrose 20 g
pH 5.7
H2O ad 1000 ml 800 ml 1600 ml
-------------------------------------------
The best combination to date appears to be
30 mM glutamine and 2 mM of methionine
.
Gelrite 1.6gr/l mg/l mg/4l mg/8l
--------------------------------------------------------------
NH4NO3 1000 4000 8000
KNO3 1500 4000 8000
Ca(NO3)2.4H20 500 2000 4000
MgSO4.7H2O 200 800 1600
KH2PO4 200 800 1600
FeEDDHA 30 120 240
KJ 0.25 1 ml 2 ml
Micro Rosa 2 ml 8 ml 16 ml
Vit Rosa 1 ml 4 ml 8 ml
CH 400 1600 3200
Inosit 100 400 800
Ade 25 100 200
Sucrose 30 g
pH 5.7
H2O ad 1000 ml 800 ml 1600 ml
.
VW 1949 + CM 15%
(NH4)2SO4 500 4 mM
KNO3 525 5
Ca3(PO4)2 200 0.6
MgSO4 122 1
KH2PO4 250 1.8
.
.
VW 2003 + CM 15%
NH4NO3 400
KNO3 600
Ca(NO3)2.4H2O 100
MgSO4.7H2O 200
KH2PO4 200
.
MICRO Pin MS B5 QL ½LM
--------------------------------------------------------------
H3BO3 3 6.2 3.0 6.2 15.0
MnSO4.1H2O 7 16.9 10 0.76 10.0
ZnNa2EDTA 2 12 2.8 12 22.0
Na2MoO4.2H2O 0.1 0.25 0.25 0.25 0.60
CuSO4.5H2O 0.05 0.025 0.025 0.025 0.25
CoCl2.6H2O 0.02 0.025 0.025 0.025 0.06
[NiCl2.6H2O] 0.01 0.01 0.01 0.01 0.01
--------------------------------------------------------------
+ KJ 0.5 0.83 0.75 0.08 2
+ Na2SiO3 10 20 20 20 20
--------------------------------------------------------------
.
.
Micro Pinus mg mg/l
-----------------------------------------------------
H3BO3 300 3
MnSO4.1H2O 700 7
ZnNa2EDTA 200 2
Na2MoO4.2H2O 10 0.1
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
Pinus Liquid mg mg/l
-----------------------------------------------------
H3BO3 200 2
MnSO4.1H2O 200 2 /4
ZnNa2EDTA 200 2
Na2MoO4.2H2O 10 0.1
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
Micro Orchids mg mg/l
-----------------------------------------------------
H3BO3 500 5
MnSO4.1H2O 1000 10
ZnNa2EDTA 500 5
Na2MoO4.2H2O 20 0.2
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
Micro Orchids Liquid
-----------------------------------------------------
H3BO3 300 3
MnSO4.1H2O 500 5
ZnNa2EDTA 500 5
Na2MoO4.2H2O 20 0.2
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
Micro Rosa mg mg/l
------------------------------------------------------
H3BO3 800 8
MnSO4.1H2O 1500 15
ZnNa2EDTA 1000 10
Na2MoO4.2H2O 25 0.25
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
Micro Holland (–Mn) Dicotyledone
------------------------------------------------------
H3BO3 800 8
MnSO4.1H2O 800 8
ZnNa2EDTA 800 8
Na2MoO4.2H2O 20 0.20
CuSO4.5H2O 5 0.05
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
Micro Holland (–Mn –B) Monootyledone [Bamboo]
------------------------------------------------------
H3BO3 300 3
MnSO4.1H2O 800 8
ZnNa2EDTA 800 8
Na2MoO4.2H2O 20 0.20
CuSO4.5H2O 10 0.10
CoCl2.6H2O 2 0.02
NiCl2.6H2O 1 0.01
H2SO4 0.1 M 1 ml
H2O ad 200 ml *2 ml
.
.
KJ mg mg/l
------------------------------------------------------
KJ 50 0.5
H2O ad 50 ml *0.5-0.3 ml
Vit Orchids Monocot mg mg/l
======================================================
Thiamine B1 100 1
Nicotinic acid NA 200 2
Pyridoxine B6 100 1
Glycine 200 2
Pantothenate Ca 200 2
Biotin, D(+) 2 0.02
Folic acid 50 0.5
H2O ad 100 *1 ml
.
.
Vit Rosa Dicot
======================================================
Thiamine B1 100 1.0
Nicotinic acid NA 50 0.5
Pyridoxine B6 50 0.5
Glycine 200 2
Ca Pantothenate 200 2
Biotin, D(+) 2 0.02
Folic acid 50 0.5
H2O ad 100 *1 ml
.
.
Vit PLB Gamborg
======================================================
Thiamine B1 1000 10.0
Nicotinic acid NA 100 1.0
Pyridoxine B6 100 1.0
Glycine 200 2
Ca Pantothenate 200 2
Biotin, D(+) 5 0.05
Folic acid 50 0.5
H2O ad 100 *1 ml
.
.
Glutamine + Vit + FILTRATION
======================================================
Glutamine 500
folic ac + ascorbic ac 5 + 1 *50 ml
.
